RESUMO
A novel technique for preparation affinity sorbent based on tyramine and tryptamine was proposed. It was shown that tryptamine-Sepharose and tyramine-Sepharose effectively bind IgG, IgA, lipoprotein (a) (Lp(a)) and low density lipoproteins (LDL) from blood plasma. The sorption capacity is 4-9 mg of IgG, 2-4 mg IgA, 3-5:mg of Lp(a) and 5-7 mg of LDL per mL of gel. It was found that new sorbents can bind Lp(a) and IgG as themselves or in a complex of Lp(a) with IgG. The existence of this complex may indicate the presence of anti-Lp(a) autoantibodies in the blood of some patients. The advantages of new sorbents are easiness of its synthesis and stability during use and storage. In practice they can be applied for medical and biotechnological purposes where it is necessary to bind Lp(a), LDL, IgG, IgA.
Assuntos
Cromatografia de Afinidade/métodos , Triptaminas/química , Tiramina/química , Humanos , Imunoglobulina A/química , Imunoglobulina A/isolamento & purificação , Imunoglobulina G/química , Imunoglobulina G/isolamento & purificação , Ligantes , Lipoproteína(a)/química , Lipoproteína(a)/isolamento & purificação , Lipoproteínas LDL/química , Lipoproteínas LDL/isolamento & purificaçãoRESUMO
Affinity haemoadsorbents based on WY, WTY, WNY ligands and polysaccharide matrix were developed for the human immunoglobulin G binding. The characteristics of new sorbents such as the binding of total IgG and binding of IgG subclasses were compared. It was found that all new sorbents well extract the IgG from the blood plasma. It was evidenced that WNY-based sorbent is more effective for binding of IgG subclass 3. The determination of physic-chemical characteristics of IgG binding revealed that desorption constants for IgG are 10 ± 3, 28 ± 4 and 13 ± 3 µM for WY, WTY, WNY based sorbents respectively. Maximum sorption capacities for IgG are 43 ± 2, 45 ± 3 and 46 ± 3 mg IgG per ml of sorbent for WY, WTY, WNY based sorbents respectively. Also it was shown that the new sorbents are compatible with blood and are suitable for the medical purposes.
Assuntos
Imunoglobulina G/sangue , Imunoglobulina G/isolamento & purificação , Oligopeptídeos/química , Hemoperfusão , Humanos , Imunoglobulina G/química , Ligantes , Estrutura Molecular , Polissacarídeos/química , Ligação Proteica , Treonina/química , Triptofano/química , Tirosina/químicaRESUMO
A method is described for simple, rapid Swan-Ganz catheter insertion. In addition, a new technique for replacement of Swan-Ganz catheters without the need for additional venipuncture is presented.
